In this application, a crude extract of norovirus virus like particle (VLP) was analyzed by a SEC/MALS method. The outside structure of a VLP mimics a virus, but it does not contain viral genetic material. Thus, it is considered as a safe and is widely used for the production of vaccines or development of drug delivery system in pathology and immunology. VLPs are often produced by microbial expression system.
The crude extract was purified by an anion exchange CIM multusTMÂ QA-1Â column. For the separation of impurities and the NVLP was achieved by using a polymer-base aqueous SEC (GFC) column, OHpak SB-805 HQ. Since UV detector cannot distinguish the impurities and the NVLP, the use of an appropriate SEC column is important. The obtained rms radius of the extract reflected the size distribution of the properly configured NVLP particle size.
Because of its sophisticated high hydrophilicity design, SB-805 HQ experiences less adsorption and have the pore size appropriate for the analysis of nanoparticles. Therefore, it is a suitable and a recommended separation tool for virus like particle VLP profiling via SEC/MALS.
Sample : 50 μL
NVLP purification Fraction
of an anion exchange chromatography
Column : Shodex OHpak SB-805 HQ (8.0 mm I.D. x 300 mm)　 Eluent : 50 mM Sodium phosphate buffer saline (pH7.5) Flow rate : 1.0 mL/min Detector : UV (280 nm), MALS Column temp. : 25 °C
