It has been reported that the anomer separation does not take place at high temperatures. Therefore, when using the SUGAR series (SP0810, SC1011, KS-801, SZ5532, or SC1211 ), set up the column temperature at 70-80 °C.
Please refer to Prevention of Anomer Separation.
Sample : 0.5% each, 10μL
Glucose, Mannose, Xylose, Galactose, Arabinose
Column : Shodex SUGAR SC1011 (8.0mmI.D. x 300mm) Eluent : H2O Flow rate : 0.7mL/min Detector : Shodex RI Column temp. : 29°C, 70°C