RSpak DE-413, a polymer-based reversed phase chromatography column, can be used with aqueous eluents with no organic solvent. In this application, simultaneous analysis of C1 compounds including formaldehyde, methanol, and formic acid was performed using an aqueous solution of phosphoric acid as an eluent.
Sample: 20 μL
2.5 mg/mL each (in H2O)
Column：Shodex RSpak DE-413 (4.6 mm I.D. x 150 mm) x 2 Eluent：10 mM H3PO4 aq. Flow rate：1.0 mL/min Detector：RI, UV (190 nm) Column temp.：50 ℃