Separation of Ceftazidime and Arginine (KW402.5-4F)

Ceftazidime is a cephem antibiotic used to treat bacterial infections. Some formulations of ceftazidime contain arginine. KW402.5-4F, an aqueous SEC (GFC) column, was used to separate ceftazidime and arginine, and a good separation was obtained.

Sample: 0.2 mg/mL each, 10 µL

1.Ceftazidime

2.L-Arginine

 	Column：Shodex KW402.5-4F (4.6 mm I.D. x 300 mm)
 	Eluent：1.15 g/L NH4H2PO4 (pH 2.0 adjusted with H3PO4)/CH3CN=25/75
 	Flow rate：0.5 mL/min
 	Detector：UV (206 nm)
 	Column temp.：30 ℃