Separation of N-acetylneuraminic Acid and N-glycolyneuraminic Acid

N-acetylneuraminic acid and N-glycolyneuraminic acid, family of sialic acid, were separated using organic-acids-analysis column,
KC-811. .

Sample : 0.1%each, 40µL

1. N-Glycolylneuraminic acid

2. N-Acetylneuraminic acid

 
 
 Column : Shodex RSpak KC-811 (8.0mmID*300mm)
 

 Eluent : 1% H3P04 aq.
 

 Flow rate : 0.1mL/min
 

 Detector : UV(210nm)
 

 Column temp. : 40° C