Separation of Substances Produced During Utilization of Biomass (VN-50 4D)

In recent years, research to utilize biomass as an energy resource has been actively conducted. The various substances such as glycerin and dihydroxyacetone are produced in the reaction process from biomass until obtaining the final product. In this application, the substances produced during biomass utilization were separated using HILICpak VN-50 4D, a polymer-based HILIC column.

Sample : 10μL
1. Dihydroxyacetone 10mg/mL
2. Glycerin 1mg/mL
3. Glyceraldehyde 0.025mg/mL
4. Glyceric acid 0.025mg/mL
5. Glycolic acid 10mg/mL
(in H₂O/CH₃CN=20/80)

Column       : Shodex HILICpak VN-50 4D (4.6mmI.D. x 150mm)
Eluent       : (A); 0.1%HCOOH, (B); CH3CN
               Linear gradient;
               (B%) 95% (0 to 2min), 95 to 20% (2 to 7min), 20% (7 to 13min)
Flow rate    : 0.6mL/min
Detector     : ELS
Column temp. : 60°C