Separation of Water-Soluble Vitamin and Sulfite (KC-811)

Simultaneous analysis of water-soluble vitamins (ascorbic acid and pantothenic acid) and sulfite was performed using RSpak KC-811 column for organic acid analysis.

Sample : 20 µL
10 μg/mL each (in eluent)
1. Ascorbic acid
2．SO₃^2- (Sodium hydrogen sulfite)
3. Pantothenic acid (Calcium pantothenate)

Column       : Shodex RSpak KC-811 (8.0 mm I.D. x 300 mm)
Eluent       : 0.1 % H3PO4 aq.
Flow rate    : 1.0 mL/min
Detector     : UV (210 nm)
Column temp. : 40 °C