Serum (YK-421)

Serum

The problems in the anaysis using IC YK-421 is
that the analysis of very small amount of NH₄⁺ in a sample which contains large amount of Na⁺ is difficult. Addition of 2.5mM 18-Crown 6-ether in 5mM Tartaric acid + 1mM Dipicolinic acid + 1.5g/L Boric acid eluent can solve the problem. Addition of crown 6-ether can adjust the elution volume of the cations. Especially, the elution volume of K⁺ is largely affected by the additon and elute after Mg²⁺.

Sample : Serum, 10?L

1. Na⁺

2. NH₄⁺

3. Ca²⁺

4. Mg²⁺

5. K⁺

 Column : Shodex IC YK-421 (4.6mmI.D. x 125mm)
 Eluent : (A) 5mM Tartaric acid + 1mM Dipicolinic acid + 1.5g/L Boric acid aq.
 (B) 5mM Tartaric acid + 1mM Dipicolinic acid + 1.5g/L Boric acid
 + 2.5mM 18-Crown 6-ether aq.
 Flow rate : 1.0mL/min
 Detector : Non-suppressed conductivity
 Column temp. : 40°C