HILICpak VC-50 2D is a polymer-based amino HILIC column that was used to analyze 20 amino acids. This method does not require the use of derivatization or ion-pair reagents. The result showed basic amino acids were separated well and tend to retain longer by the carboxyl functional group modified on VC-50 2D column packing material. When analyzing acidic amino acids, we recommend HILICpak VG-50 2D and Asahipak NH2P-40 2D.
Refer to:
Simultaneous Analysis of Saccharides, Organic Acids and Amino Acids with LC/MS (VG-50 2D)
LC/MS Analysis of Essential Amino Acids (NH2P-40 2D)
Sample : Amino acid standards 0.1μg/mL each (in H2O), 5μL
| Aspartic acid, Asp | Glutamic acid, Glu | Cysteine, Cys | Threonine, Thr |
| Serine, Ser | Proline, Pro | Tyrosine, Tyr | Asparagine, Asn |
| Methionine, Met | Glutamine, Gln | Lysine, Lys | Alanine, Ala |
| Glycine, Gly | Phenylalanine, Phe | Valine, Val | Leucine, Leu |
| Isoleucine, Ile | Tryptophan, Trp | Histidine, His | Arginine, Arg |
Column : Shodex HILICpak VC-50 2D (2.0mmI.D. x 150mm)
Eluent : H2O/100mM HCOOH aq./CH3CN
=30/20/50 to 0/90/10 (0 to 5min), 0/90/10 (5 to 30min)
(Low pressure linear gradient)
Flow rate : 0.2 mL/min
Detector : ESI-MS SIM
Column temp. : 30℃
