Simultaneous Analysis of Lactose, Epilactose, and Lactulose (VG-50 4E)

Lactose, lactulose, and epilactose were analyzed by HILIC mode simultaneously using polymer-based amino column HILICpak VG-50 4E.

Sample : 5mg/mL each, 5uL

Column       : Shodex HILICpak VG-50 4E (4.6mmI.D. x 250mm)
Eluent       : CH3CN/CH3OH/H2O=75/20/5
Flow rate    : 1.0mL/min
Detector     : Shodex RI
Column temp. : 40°C