Nitrous acid and ammonium thiocyanate have been known to cause germination failure in plants. In this application, a standard solution of nitrous acid and ammonium thiocyanate was analyzed using Asahipak NH2P-50 4E, a polymer-based amino column described in the 2016 testing methods for fertilizers. It was confirmed that the calibration curve of nitrous acid and ammonium thiocyanate shows good linearity respectively in the range from 1 to 20μg/mL.
Sample : 10μg/mL each, 10μL
Column : Shodex Asahipak NH2P-50G 4A (4.6mmI.D. x 10mm) + NH2P-50 4E (4.6mmI.D. x 250mm) Eluent : Na2HPO4·12H2O (1.79g) + NaH2PO4·2H2O (0.78g) + NaClO4·H2O (14.04g) in H2O (1000mL) Flow rate : 1.0mL/min Detector : UV(210nm) Column temp. : 40°C