Ten kinds of water-soluble vitamins were simultaneously analyzed using RSpak DE-413, a polymer-based reversed phase chromatography column.
Note: we recommend 2X DE-413 in tandem for better simultaneous separation.
Sample : 10 μL
1. Vitamin B1 200 µM
2. Vitamin B6 200 µM
3. Niacinamide 200 µM
4. Vitamin C 200 µM
5. Vitamin B3 200 µM
6. Vitamin B5 200 µM
7. Vitamin B12 200 µM
8. Vitamin B2 200 µM
9. Biotin 800 µM
10. Folic acid 20 µM
Columns : Shodex RSpak DE-G 4A (4.6 mm I.D. x 10 mm) + DE-413 (4.6 mm I.D. x 150 mm) Eluent : (A); 10 mM H3PO4 aq./(B); CH3CN High pressure linear gradient; (B %) 0 % to 20 % (0 to 15 min), 20 % (15 to 16 min), 0 % (16.01 to 25 min) Flow rate : 1.0 mL/min Detector : PDA (190 - 400 nm) Column temp. : 40 ℃