Simultaneous Analysis of Water-Soluble Vitamins (DE-413)

Ten kinds of water-soluble vitamins were simultaneously analyzed using RSpak DE-413, a polymer-based reversed phase chromatography column.

Note: we recommend 2X DE-413 in tandem for better simultaneous separation.

Sample : 10 μL

1. Vitamin B₁ 200 µM
2. Vitamin B₆ 200 µM
3. Niacinamide 200 µM
4. Vitamin C 200 µM
5. Vitamin B₃ 200 µM
6. Vitamin B₅ 200 µM
7. Vitamin B₁₂ 200 µM
8. Vitamin B₂ 200 µM
9. Biotin 800 µM
10. Folic acid 20 µM

Columns      : Shodex RSpak DE-G 4A (4.6 mm I.D. x 10 mm) + DE-413 (4.6 mm I.D. x 150 mm)
Eluent       : (A); 10 mM H3PO4 aq./(B); CH3CN
               High pressure linear gradient;
               (B %) 0 % to 20 % (0 to 15 min), 20 % (15 to 16 min), 0 % (16.01 to 25 min)
Flow rate    : 1.0 mL/min
Detector     : PDA (190 - 400 nm)
Column temp. : 40 ℃