With SUGAR SH1011 and SH1821, which operate under ion exclusion and GFC modes, the use of perchloric acid aqueous solution allows analysis of saccharides and organic acids in a single run. By changing the concentration (pH) of the perchloric acid aqueous solution and the column temperature, the elution time of organic acids can be adjusted. However, when using columns such as SH1011 and SH1821 whose counter ions are of H+ type for the analysis of sucrose in a sample, measurement should be performed at a temperature as low as around 15°C for preventing the decomposition of sucrose.
Sample: 20-hold diluted Soft Drink
4. Citric acid
7. Succinic acid.
Column : Shodex SUGAR SH1011 (8.0mmI.D. x 300mm) Eluent : 3mM HClO4 aq. Flow rate : 1.0mL/min Detector : Shodex RI Column temp. : 15°C