The column shipping solvent of EV-2000 column is ethyl acetate/cyclohexane(3/7).
(1) After pumping acetone/cyclohexane (2/8) at 2mL/min for 2h.
(2) After pumping ethyl acetate/cyclohexane (2/8) at 2mL/min for 2h.
(3) After pumping acetone/cyclohexane (2/8) at 2mL/min for 2h.
(4) After pumping ethyl acetate/cyclohexane (2/8) at 2mL/min for 2h.
Sample :
1. Corn oil
2. Fluvalinate
3. Chinomethionate
Columns : Shodex CLNpak EV-G (20mmID*100mm) + EV-2000 (20mmID*300mm) Flow rate : 4.0mL/min Detector : UV(254nm) Column temp. : 25°C