When analyzing saccharides in food, it is necessary to remove the substances besides saccharides before the analysis. This procedure is called as sample pretreatment.
Here, the analysis result of saccharides in food using SUGAR SP0810 is shown together with the sample pretreatment procedure.
(Sample pretreatment for soybean flour)
1) Put the soybean flour in a beaker and add pure water to make a 50-fold solution.
2) Extract the substances from the paste into the water using an ultrasonic vibrator for 30 minutes.
3) Perform ultrafiltration (MW : 10,000).
4) Pass through a 0.45μm membrane filter before injecting into HPLC.
Sample: Soybean Flour
1. Stachyose
2. Raffinose
3. Sucrose
4. Maltose
5. Glucose.
Columns : Shodex SUGAR SP-G 6B (6.0mmI.D. x 50mm) + SP0810 (8.0mmI.D. x 300mm) x 2 Eluent : H2O Flow rate : 0.4mL/min Detector : Shodex RI Column temp. : 80°C
