Soysaponin in Soy Beans (DS-613)

Soysaponin in soy bean were analyzed using RSpak DS-613 (a column for reversed phase chromatography).

Sample :
1-14. Unknown
15. Soysaponin Bb
16-21. Unknown

 Column : Shodex RSpak DS-613 (6.0mmID*150mm)
 Eluent : CH3COOH/H2O/Isopropanol/CH3CN=0.1/63.4/4.2/32.3
 Flow rate : 1.0mL/min
 Detector : UV(205nm)
 Column temp. : 25 to 27°C