HILICpak VC-50 2D was used to separate trivalent chrome (Cr3+) and hexavalent chrome (CrO42-). The packing material is made from polyvinyl alcohol base material modified with carboxyl functional group is packed in VC-50. In this analysis, trivalent chrome is retained mainly by ion-exchange effects while hexavalent chrome is mainly retained by hydrophobic interaction and they were well separated. The linearity of calibration curves for both ions were confirmed.
Sample : 10 μg/L each, 25 μL
1. Cr(III) from Cr(NO3)3
2. Cr(VI) from K2CrO4
*diluted with eluent
Column : Shodex HILICpak VC-50 2D (2.0 mm I.D. x 150 mm) Eluent : 9 mM HNO3 aq. Flow rate : 0.2 mL/min Detector : ICP-MS SIM m/z 52 *Helium was used as reagent gas Column temp. : 40 °C