Standard Proteins (25) (KW403-4F)

Ten kinds of protein standards were analyzed using KW403-4F (a semi-micro column for aqueous SEC (GFC)).

Sample: 5μL

1. Thyroglobulin (MW: 669,000)                      6. β-Lactoglobulin (MW: 35,000)

2. γ-Globulin (MW: 158,000)                             7. Myoglobin (MW: 17,000)

3. BSA (MW: 67,000)                                           8. Ribonuclease A (MW: 13,700)

4. Ovalbumin (MW: 43,000)                              9. Aprotinin (MW: 6,500)

5. Peroxidase (MW: 40,200)                              10. Vitamin B₁₂ (MW: 1,350)

Column       : Shodex KW403-4F (4.6mmI.D. x 300mm)
Eluent       : 50mM Sodium phosphate buffer(pH7.0) + 0.3M NaCl
Flow rate    : 0.33mL/min
Detector     : UV(220n