Standard Proteins (27) (KW405-4F)

Ten kinds of protein standards were analyzed using KW405-4F
(a semi-micro column for aqueous SEC(GFC)).

Sample : 5micro-L

1. Thyroglobulin (MW: 669,000)

2. gamma-Globulin (MW: 158,000)

3. Ovalbumin (MW: 43,000)

4. Ribonuclease A (MW: 13,700)

5. Aprotinin (MW: 6,500)

Column : Shodex KW405-4F (4.6mmID*300mm) 

 Eluent : 50mM Sodium phosphate buffer(pH7.0) + 0.3M NaCl 

 Flow rate : 0.33mL/min 

 Detector : UV(220nm) (small cell volume) 

 Column temp. : 30deg-C