Steroids (SB-802.5 HQ)

Three kinds of steroids were separated using OHpak SB-802.5 HQ (a column for GFC separation).

Sample : 0.01% each, 20μL
1. Cortisone
2. Prednisolone
3. Testosterone

Column       : Shodex OHpak SB-802.5 HQ (8.0mmI.D. x 300mm)
Eluent       : H2O/CH3CN=60/40
Flow rate    : 1.0mL/min
Detector     : UV(240nm)
Column temp. : 70°C