Stevioside and Rebaudioside A (NH2P-50 4E)

Polymer-based-amino column, Asahipak NH2P-50 4E was used to separate stevioside and rebaudioside A, which were extracted from stevia.

Sample : 0.05% each, 20μL
1. Stevioside
2. Rebaudioside A

Column       : Shodex Asahipak NH2P-50 4E (4.6mmI.D. x 250mm)
Eluent       : H2O/CH3CN=25/75
Flow rate    : 1.0mL/min
Detector     : UV(210nm)
Column temp. : 30°C