is for the separation of sugars using ligand-exchange and normal-phase modes.
The ligand used is Na, thus even for the samples containing sodium salt does not
require desalting sample preparation steps. The samples treated with acids can
be analyzed after neutralizing with sodium hydroxide. *DC-613 sugar analysis
requires mobile phase with high concentration of ACN, thus samples containing
a large amount of salt may cause precipitation.
Sample : 10µL, 0.1% each
Column : Shodex RSpak DC-613 (6.0mmID*150mm)
Eluent : CH3CN/H2O=70/30
Flow rate : 0.8mL/min
Detector : Shodex RI
Column temp. : 50° C