IC YK-421. By using the mobile phase used below (high ACN concentration), hydrophobic interaction is suppressed. Thus ioninc-interaction dominates, this makes shorter chain alkylammonium to be retained longer than longer ones.
Sample : 100mg/L each, 20?L
1. Tetrahexylammonium
2. Tetrapentylammonium
3. Tetrabutylammonium
4. Tetrapropylammonium
5. Tetraethylammonium
6. Tetramethylammonium
Column : Shodex IC YK-421 (4.6mmID*125mm) Eluent : 2.5mM HNO3 aq./CH3CN=30/70 Flow rate : 1.0mL/min Detector : Shodex CD Column temp. : 40°C