Tetracaine was analyzed using Asahipak ODP-50 4D, a column for reversed-phase chromatography, with eluents of different pH values.
Sample : Tetracaine hydrochloride
Column : Shodex Asahipak ODP-50 4D (4.6mmI.D. x 150mm) Eluent : 25mM Phosphate buffer/CH3CN Flow rate : 0.6mL/min Detector : UV(254nm) Column temp. : 30°C