Asahipak NH2P-40 3E is filled with smaller-sized gel, 4 um, and packed in a 3.0-mm internal diameter (ID) column housing. The selected ID size allows the column to be used with a conventional LC system, i.e., without a need of semi-micro or any other special LC system. NH2P-40 3E provides higher resolution compared to Asahipak NH2P-50 4E. NH2P series is one of the best fits for the saccharides analysis using HILIC mode. The solvent required for NH2P-40 3E column is approximately 1/3 of the NH2P-50 4E column.
Sample: Sample : 0.5% each, 4µL
1. Fructose
2. Glucose
3. Sucrose
| ? | NH2P-40 3E | NH2P-50 4E | |
| TPN (Sucrose) | 11,300 | 8,700 | |
| Rs | Fructose/Glucose | 6.4 | 4.2 |
| Glucose/Sucrose | 8.5 | 5.9 | |
Data obtained by a conventional RI (0.25 mm ID tubing).
Columns : Shodex Asahipak NH2P-40 3E (3.0mmID*250mm), NH2P-50 4E (4.6mmID*250mm) Eluent : H2O/CH3CN=25/75 Flow rate : (NH2P-40 3E) 0.35mL/min, (NH2P-50 4E) 1.0mL/min Detector : Shodex RI Column temp. : 25°C
