IC YK-421. By using the mobile phase stated below, hydrophobic interaction dominates ioninc-interaction. Therefore, shorter chain alkyl amines (less hydrophobic) will elute before longer ones.
Sample : Alkylamines 100mg/L, 20?L
Column : Shodex IC YK-421 (4.6mmID*125mm) Eluent : 4mM HNO3 aq./CH3CN=70/30 Flow rate : 1.0mL/min Detector : Shodex CD Column temp. : 40°C