Trypsin and Trypsinogen

Two trypsins, alpha and beta were separated using HIC PH-814 ( a hydrophobic interaction column ).

Sample :
1. alpha Trypsin
2. Trypsinogen
3. beta Trypsin

 Column : Shodex HIC PH-814 (8.0mmID*75mm)
 Eluent : (A); (B) + 1.8M Ammonium sulfate
 (B); 0.1M Phosphate buffer(pH3.0)
 Linear gradient: 0min to 60min, (A) to (B)
 Flow rate : 1.0mL/min
 Detector : UV(280nm)
 Column temp. : 25°C