Hemoglobin A, F, S and C were analyzed using IEC SP-FT 4A, a non-porous type column for cation exchange chromatography. SP-FT 4A can provide ultra-rapid and high-resolution analysis using conventional devices as same as when using UHPLC.
(Caution) Backpressure will differ depends on the LC system used and the analytical conditions such as eluent compositions and column temperature. Please set the system pressure (sum of column and system pressures) less than 20 MPa.
Sample : 5μL
AFSC Hemoglobin Control
(51-fold diluted)
Column : Shodex IEC SP-FT 4A (4.6mmI.D. x 10mm)
Eluent : (A); 20mM *MES buffer(pH5.6)
(B); (A) + 0.5M Na2SO4
Linear gradient: 5% (B) to 100% (B) (2min)
Flow rate : 1.7mL/min
Detector : VIS(415nm)
Column temp. : 30°C
*MES : 2-(N-Morpholino) ethanesulfonic acid
