Unsaturated fatty acids are expected to prevent arteriosclerosis and thrombosis, and improve visual function and allergy symptoms. In this application, three kinds of Omega-3 fatty acids and two kinds of Omega-6 fatty acids were analyzed using Asahipak ODP-50 4D, a polymer-based reversed phase chromatography column.
Sample : 5uL
0.002% each (in Ethanol)
1. EPA (Eicosapentaenoic acid)
2. α-Linolenic acid
3. DHA (Docosahexaenoic acid)
4. Arachidonic acid
5. Linoleic acid
Column : Shodex Asahipak ODP-50 4D (4.6mmI.D. x 150mm) Eluent : 0.1% H3PO4 in (H2O/CH3CN=30/70) Flow rate : 1.0mL/min Detector : UV(215nm) Column temp. : 40°C
