Vitamin B12 & Creatinine (SB-802.5 HQ)

This shows an analysis example of vitamin B₁₂ together with creatinine and urea by reversed phase mode using OHpak SB-802.5 HQ.

Sample : 10 μL
1. Creatinine 100 μg/mL
2. Urea 2000 μg/mL
3. Vitamin B₁₂ 200 μg/mL
*dissolved in eluent

Column       : Shodex OHpak SB-802.5 HQ (8.0 mm I.D. x 300 mm)
Eluent       : 10 mM H3PO4 aq.
Flow rate    : 1.0 mL/min
Detector     : UV (210 nm)
Column temp. : 40 °C