Vitamin C (2) (NH2P-50 4E)

For the analysis of ascorbic acid, amino or silica based columns are usually used. Analysis using amino columns is particularly wide spread because isoascorbic acid as well as ascorbic acid can be analyzed together.
However, conventional amino columns are silica-based, bonded with aminopropyl groups or polyamine and are not chemically stable. Therefore, they have certain disadvantage such as a decrease in retention and short column life. Since Asahipak NH2P-50 4E is a polymer-based column, it does not suffer from such disadvantages. Moreover, since NH2P-50 column can be washed with alkaline solvents, the column life can be greatly extended.

Sample : (left) standards, (left) Grapefruit juice
1. D-Isoascorbic acid
2. L-Ascorbic acid

 Column : Shodex Asahipak NH2P-50 4E (4.6mmID*250mm)
 Eluent : 60mM Phosphoric acid(pH2.0)/CH3CN=20/80
 Flow rate : 1.0mL/min
 Detector : UV(254nm)
 Column temp. : 40°C

Equilibration of NH2P-50 4E column : After passing 60mM Phosphoric acid in the column by 0.5 mL/min for 2 hours, it replaces by eluent.