However, conventional amino columns are silica-based, bonded with aminopropyl groups or polyamine and are not chemically stable. Therefore, they have certain disadvantage such as a decrease in retention and short column life. Since Asahipak NH2P-50 4E is a polymer-based column, it does not suffer from such disadvantages. Moreover, since NH2P-50 column can be washed with alkaline solvents, the column life can be greatly extended.
Sample : (left) standards, (left) Grapefruit juice
1. D-Isoascorbic acid
2. L-Ascorbic acid
Column : Shodex Asahipak NH2P-50 4E (4.6mmID*250mm) Eluent : 60mM Phosphoric acid(pH2.0)/CH3CN=20/80 Flow rate : 1.0mL/min Detector : UV(254nm) Column temp. : 40°C
Equilibration of NH2P-50 4E column : After passing 60mM Phosphoric acid in the column by 0.5 mL/min for 2 hours, it replaces by eluent.