Xylitol is officially permitted as a food additive by the Ministry of Welfare in Japan on April 17, 1997. The chromatogram is an analysis of xylitol in tablet candy. Asahipak NH2P-50 4E column is quite useful for the analysis of sugar alcohols including xylitol. NH2P-50 is a polymer-based amino column packed with the polyvinyl alcohol gel bonded with a polyamine. It is good for quantitative analysis and can be washed with alkaline solvents.
2% aqueous solution of the sample was passed through a 0.45μm filter,
and the same volume of acetonitrile was added.
Sample: Tablet candy, 20μL
Column : Shodex Asahipak NH2P-50 4E (4.6mmI.D. x 250mm) Eluent : H2O/CH3CN=25/75 Flow rate : 1.0mL/min Detector : Shodex RI Column temp. : 30°C