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Trending Applications: What’s new

Saccharides

Rapid Analysis of Mannose and Lactose (VG-25 2B)

Sep 17, 20261 min read

Shodex HILICpak VG-25 2B is a compact semi-micro HILIC column for rapid sugar analysis. In this application, mannose and lactose were separated in less than 6 minutes using isocratic acetonitrile/water conditions, demonstrating fast analysis on the 50 mm column format. […]

Saccharides

Rapid Analysis of Fructose, Glucose, and Sucrose (VG-25 2B)

Sep 17, 20261 min read

Shodex HILICpak VG-25 2B is a semi-micro HILIC column designed for the analysis of sugars and sugar alcohols. The column has a 2.0 mm I.D. × 50 mm format with 2.5 µm polymer-based amino-modified packing material. In this application, fructose, […]

Proteins, Peptides and Amino Acids

PEG-Chymotrypsin (KW-802.5)

Sep 11, 20261 min read

Modification of protein is one of research methods of protein function. Functional performance or stability is changed by the modification. PEG modified chymotrypsin is analyzed using PROTEIN KW-802.5. Sample : 50 μL PEG-Chymotrypsin 0.1 % Column : Shodex PROTEIN […]

Anions (LC/MS)

LC/MS/MS Analysis of Phosphonic Amino Acid Herbicides and Their Metabolites, including Glyphosate, Referencing to China National Standard GB 23200.122-2026 (SI-50 4D)

Aug 31, 20262 min read

In this application, LC/MS/MS analyses of four phosphonic acid amino acid herbicides, including glyphosate and glufosinate, and six of their metabolites were performed using IC SI-50 4D. National Standard of China GB 23200.122-2026*, “National food safety standard – Determination of […]

Reversed Phase Analysis

LC/MS/MS Analysis of PFAS Based on the Korean Guideline for the Analysis of Prohibited Ingredients in Cosmetics (C18U 2B)

Aug 31, 20262 min read

The Guideline for the Analysis of Prohibited Ingredients in Cosmetics (화장품 중 배합금지성분 분석법 가이드라인), published in Korea in 2024, includes analytical methods for PFAS compounds as well as tetraconazole, a fluorinated pesticide. In this application, six PFAS compounds that […]

Saccharides

Analysis of Sugars Using Ethanol: A Less Toxic Alternative to Acetonitrile (2) (VG-50 4D)

Aug 31, 20261 min read

Acetonitrile is commonly used in HILIC applications; however, it is classified as a hazardous substance. In contrast, ethanol is known to have lower toxicity than many other organic solvents. In this application, sugars were analyzed using commercially available 95% ethanol […]

News

Oct 6, 2026

LB Series Columns Sales Suspended

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Sep 14, 2026

Sugar & Carbohydrate Analysis by HPLC: Columns, Methods and Applications

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May 12, 2026

PFAS Analysis by LC–MS/MS Using Shodex Columns

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Apr 24, 2027

2027 Pittcon Conference and Exhibition

April 24, 2027 – April 28, 2027 @ 9:00 am – 5:00 pm – Visit the Shodex HPLC columns team at Booth 1423 at the conference and exhibition. More information including poster abstracts are coming soon.

May 31, 2026

74th ASMS Conference on Mass Spectrometry and Allied Topics

May 31, 2026 – June 4, 2026 @ 9:00 am – 5:00 pm – Exhibit Booth 722 Visit us at Booth 722 to learn more about how Shodex LC columns can move your mass spectrometry projects forward. More info coming…

Mar 9, 2026

Pittcon 2026 Conference & Exposition

March 9, 2026 – March 11, 2026 @ 10:00 am – 5:00 pm – Meet the Shodex Team at Booth 1117 We are pleased to have a booth and poster (Session Number: PS-P106) at Pittcon 2026 in San Antonio. Please…

Applications

GPC Clean-Up of Pesticides Residuals in Foods

Separation with Acetone/Cyclohexane Eluent (EV-2000 AC)

ByRonMar 23, 2015

CLNpak EV-2000 AC is used for GPC clean-up of pesticides residuals in food with acetone/cyclohexane eluent. Sample : 1. Palmitic acid, 2. Oleic acid 3. Linolenic acid, 4. Fluvalinate 5. Chinomethionate, 6. beta-Carotene 7. Methoprene, 8. Cyhalothrin 9. Cyfluthrin,…

Purine Bases, Pyrimidine Bases, Nucleosides and Nucleotides

Guanine and Tryptophan (DM-614, NN-814 and DE-613)

Mar 23, 2015

Guanine and tryptophan were analyzed using three columns for reversed phase chromtography, RSpak DM-614, NN-814 and DE-613. Sample : 1.…

Hormones

Steroids (SB-802.5 HQ)

Mar 23, 2015

Three kinds of steroids were separated using OHpak SB-802.5 HQ (a column for GFC separation). Sample : 0.01% each, 20μL…

Saccharides and Organic Acids

Fermentation By-products (Model Solution)

Mar 23, 2015

SUGAR SH1011 is used for samples of low molecular weight and SUGAR SH1821 packed with the material of larger pore…

Effects of Column Unit on Separation (HK-404L: PS)

Jul 13, 2022

Polystyrene (Mp 580) was analyzed using GPC HK-404L, an organic SEC (GPC) column for ultra-rapid analysis. The different numbers of…

Proteins, Peptides and Amino Acids GFC Analysis

Molecular Weight Measurements of Low Molecular Weight Heparins According to USP-NF GENERAL CHAPTERS (KW-803 + KW-802.5)

Feb 22, 2024

The GENERAL CHAPTERS of the United States Pharmacopeia and the National Formulary (USP-NF 2023 ISSUE 3*) includes “<209> Low Molecular…

Ion Exchange Analysis

Effect of Sample Load on Peak Width (SP-825)

Mar 23, 2015

When IEC SP-825 (a column for strong cation exchange chromatography) is used, the peak does not widen until the sample…

Hydrophobic Polymers: Other Eluents

Biodegradable Polymer (5) (Poly(Lactic-co-Glycolic Acid)) (KF-804L)

Feb 17, 2021

A poly(lactic-co-glycolic acid) (PLGA), one of biodegradable polymers, was analyzed using GPC KF-804L (a column for organic SEC (GPC)). Sample…

Monovalent and Divalent Cation Standards (8) (Ethylenediamine, EDA)

Mar 23, 2015

Ethylenediamine (0.1% v/v of 50 mg/mL) is added to water samples to prevent HCl production from the reaction between residual…

Anions(Non-suppressor Method: I-524A Column)

Analysis of Hydrophobic Anions (I-524A)

Mar 23, 2015

Analysis of Hydrophobic Anions (I-524A) Four hydrophobic anions were analyzed using an anion analysis column for non-suppressor method, IC I-524A.…

Recent Applications

Rapid Analysis of Mannose and Lactose…

Sep 17, 2026

Rapid Analysis of Fructose, Glucose, and…

Sep 17, 2026

PEG-Chymotrypsin (KW-802.5)

Sep 11, 2026

Latest Posts

Rapid Analysis of Mannose and Lactose (VG-25 2B)

Sep 17, 2026

Rapid Analysis of Fructose, Glucose, and Sucrose (VG-25 2B)

Sep 17, 2026

PEG-Chymotrypsin (KW-802.5)

Sep 11, 2026

LC/MS/MS Analysis of Phosphonic Amino Acid Herbicides and Their Metabolites, including Glyphosate, Referencing to China National Standard GB 23200.122-2026 (SI-50 4D)

Aug 31, 2026

LC/MS/MS Analysis of PFAS Based on the Korean Guideline for the Analysis of Prohibited Ingredients in Cosmetics (C18U 2B)

Aug 31, 2026

Analysis of Sugars Using Ethanol: A Less Toxic Alternative to Acetonitrile (2) (VG-50 4D)

Aug 31, 2026

Anion Standards and Organic Acids (4) (SI-50 4D)

Aug 31, 2026

Analysis of TMAH and Cations (YS-50)

Aug 31, 2026

Comparison of Separation Performance between 2-MeTHF and THF Eluents in SEC Analysis of Standard Polystyrene (KF-802)

Aug 31, 2026

Effects of Methanesulfonic Acid Concentration on Elution Time (YS-50)

Aug 28, 2026

Trending Applications: What’s new

Anions (Suppressor Method: SI-90, SI-50, SI-52, SI-35, SI-36, SI-37 Columns)

Anion Standards and Organic Acids (4) (SI-50 4D)

Aug 31, 20261 min read

Please use this column with suppressor type ion chromatography system. Anion standards and organic acids were analyzed using IC SI-50 4D (a column for anions analysis). Sample: 20 µL F– 2 mg/L Acetate 10 mg/L Formate 2 mg/L Cl– 3 mg/L NO2– 5 […]

Cations (Non-suppressor Method: YS-50 Column)

Analysis of TMAH and Cations (YS-50)

Aug 31, 20261 min read

Tetramethylammonium hydroxide (TMAH) is widely used as a photoresist developer in semiconductor manufacturing processes. In photoresist developer solutions, trace levels of cations such as Na+, NH4+, and K+ must be carefully controlled. In this application, standard solutions of TMAH, Na+, NH4+, […]

Hydrophobic Polymers: THF Eluent

Comparison of Separation Performance between 2-MeTHF and THF Eluents in SEC Analysis of Standard Polystyrene (KF-802)

Aug 31, 20262 min read

Tetrahydrofuran (THF) is a widely used mobile phase in organic solvent-based size exclusion chromatography (SEC) due to its excellent solubility for a broad range of polymeric materials. In recent years, growing interest in green chemistry has driven the search for […]

Cations (Non-suppressor Method: YS-50 Column)

Effects of Methanesulfonic Acid Concentration on Elution Time (YS-50)

Aug 28, 20261 min read

Using IC YS-50, changing the concentration of methanesulfonic acid in the eluent enables to control cations separation and retention time. Although, retention time of cations will become short if concentration is high, the eluent conductivity (background level) becomes also high. […]

Saccharides

Analysis of Glucose According to National Standards of China GB/T 20882.1-2025 (SH1011/SC1011)

Aug 3, 20262 min read

The National Standards of China GB/T 20882.1-2025*, Quality requirements for starch sugar – Part 1: Edible dextrose, lists two HPLC methods for the determination of glucose content. *The version at the time of the application acquisition. Chromatographic Condition 1 A […]

Saccharides and Sweetning agents(Additives for Foods)

Analysis of D-Allulose According to Chinese “Three New Foods” Announcement (No. 4, 2025*) (SC1011)

Aug 3, 20261 min read

In accordance with the provisions of the “Food Safety Law of the People’s Republic of China,” D-allulose has been approved as a new food ingredient. For the “Assay” test with liquid chromatography of D-allulose, the method specifies the use of […]

Latest Posts

LC/MS/MS Analysis of Phosphonic Amino Acid Herbicides and Their Metabolites, including Glyphosate, Referencing to China National Standard GB 23200.122-2026 (SI-50 4D)

Aug 31, 2026

LC/MS/MS Analysis of PFAS Based on the Korean Guideline for the Analysis of Prohibited Ingredients in Cosmetics (C18U 2B)

Aug 31, 2026

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