Shodex Column Alternatives for Organic Acid and Carbohydrate Analysis

Record: 696

Category: Column Selection
Product: DE-413, SC1011, SC1211
Sample: N/A

Question
Do you have Shodex column alternatives for Thermo Acclaim OA and Agilent Hi-Plex Ca columns?

Answer

Yes. Shodex offers columns that may be appropriate alternatives depending on the target analytes and separation mechanism.

For applications similar to Thermo Acclaim OA, Shodex DE-413 is generally the closest match in application space for aqueous analysis of organic acids and other small polar compounds. Both columns are used with aqueous acidic mobile phases, where separation is governed primarily by ion-exclusion and hydrophilicity rather than ligand exchange. If the customer’s specific target analytes are not confirmed, Shodex recommends verifying the analyte list and sample matrix before final column selection.

For applications similar to Agilent Hi-Plex Ca, Shodex offers SUGAR SC1011 and SUGAR SC1211, which are Ca²⁺-form cation-exchange sugar columns for neutral carbohydrate analysis. SUGAR SC1011 separates sugars using a mixed mode of ligand exchange and size exclusion and is commonly used for mono- and disaccharides and sugar alcohols under fully aqueous conditions. SUGAR SC1211 uses a mixed mode of ligand exchange and hydrophilic interaction and is typically applied using water/acetonitrile mobile phases. In practice, SC1011 is typically selected for fully aqueous, high-temperature sugar separations, while SC1211 is commonly used when water/acetonitrile conditions are preferred or when additional selectivity for sugar alcohols is needed.

Because these column families rely on different separation mechanisms, confirmation of the customer’s specific analytes and method conditions is recommended before final column selection.

Recommended applications:

• Elution Volume of Organic Acids (RSpak DE-413)
• Column Selection: by Combination of Saccharides

Additional Comments

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