column information

Record: 467

Category: Column Selection
Product:
Sample:

Question
Requirements;
Target proteins: 20-200KDa
Mobile phase: aqueous buffer pH 4-11

Answer
We would like to recommend our GS series GFC column which is suitable for the analysis under widest pH range.

Asahipak GS-520 HQ
pH 2-12
Max Pressure 30 bar.
Exclusion limit (pullulan) 300K Da, maximum pore size 2000A.
http://shodex.net/index.php?seitenid=1&reiter=r_results&prodid=40

One example we have is below, please note the long analysis time is due to the use of 4 columns in series, which probably is not required in your case.
http://shodex.net/index.php?seitenid=1&reiter=r_results&applic=404#
http://shodex.net/index.php?seitenid=1&reiter=r_results&applic=405

Recovery information.
http://shodex.net/index.php?seitenid=1&reiter=r_results&applic=572

Below is not an application of GS column, but GF series which is very similar to GS.
http://shodex.net/index.php?seitenid=1&reiter=r_results&applic=584
It says that KW column (another GFC column) provides the best separation, however this is a silica based column and can only be used in the pH range 3-7.5 and that of GF is 2-9. I think we can expect similar separation with GS-520.

2. Some information on SI-90 and SI-52 anion exchange columns.

SI-90:
http://shodex.net/index.php?seitenid=1&reiter=r_results&applic=710&prodid=6
Common anion analysis
http://shodex.net/index.php?seitenid=1&reiter=r_results&applic=690

SI-52:
http://shodex.net/index.php?seitenid=1&reiter=r_results&prodid=8

Analysis of anion and oxyhalides
http://shodex.net/index.php?seitenid=1&reiter=r_results&applic=710
http://shodex.net/index.php?seitenid=1&applic=1328

 
 
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