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Sugar Column Selection for RI Detection of Common Mono- and Disaccharides

Record: 623

Category: Column Selection
Product: SC1011 or SP0810
Sample: glucose, fructose, sucrose, xylose, and galactose

Question

I need a sugar column for the analysis of glucose, fructose, sucrose, xylose, and galactose using a refractive index (RI) detector. Do you have a column recommendation?

Answer

Several Shodex sugar columns may be suitable for this analysis; however, the optimal choice depends on additional details about the sample and method conditions. In particular, information such as sample matrix, presence of acids or salts, desired resolution between glucose and fructose, and mobile-phase composition can significantly influence column selection.

Both Ca²⁺-form, such as SC1011, and Pb²⁺-form ligand-exchange sugar columns, such as SP0810, are commonly used for RI-based sugar analysis. Ca²⁺-form columns are generally more tolerant of real sample matrices and are widely applied in routine food and carbohydrate analysis, while Pb²⁺-form columns can offer different selectivity but require samples to be free of acids, chelating agents, and other ionic species. Without additional method details, it is not possible to recommend a single column with certainty.

As a reference, the application below illustrates the elution behavior of common mono- and disaccharides, including glucose, fructose, sucrose, galactose, and xylose, and can be used to evaluate relative retention trends when selecting a ligand-exchange sugar column.

Recommended Application:

Once details such as sample composition, eluent conditions, and required resolution are clarified, a more specific column recommendation can be provided. For tailored technical support, please contact Shodex Technical Support at support@shodexhplc.com.

Additional Comments

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