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Columna de Gonher

Record: 360


Category: Column Problem
Product: C18
Sample:


Question
In this case the customer make the clean to the column with ACN 100% at 50ºC, but the column generates first broad peaks and then split peaks.
What can we do?

 
 

Answer
I’m sorry to say but I don’t think the column can be regenerated at this point. The main problem seems like the sample preparation.
I am not sure how many injections they made, but as written in the article I indicated before, if proper sample preparation was not done, only 40 injection can damage the column and there’s no way to restore it.

 
 
Additional Comments

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