Equivalent columns for Flowscience request

Record: 26

Category: Column Selection
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Question
looking for equivalent columns for various C18, C8, and CN columns

Answer
1. C18 RP 18 – 5µm 125 x 4,0 mm. HPLC Reverse fase 100A poro, Area is 350m2/g , 21,0% carbon wich is an equivaken column with a HPLC EliteLechrom.

*Shodex C18-D (5um, 4.6 x 150, 17% C, A=320m2/g, 12nm A pore)
There will be less retention (less C load, but the analysis maybe bit longer, longer column length)

2. C18 RP18e – 5µm – 125 x 4,0mm. 120A pore size , 330m2/g Area , 17% de carbon. a pH bwt 1,5 to 10,5.

*RSpark RP18-413 (3.5 um, 4.6 x 150, no functional group – but will work like a reversed phase mode, 200 A pore)
Only reversed phase column that is usable under pH bt 1-13.
But since this is not modified with any carboxyl functional group, the separation performance may differ from expected RP analysis.

If the customer is not using mobile phase <2, ODP50 4D (5 um, 4.6 x 150 mm, 250 A pore, pH 2-13) can be recommended. This has octadecyl functional group. Also this has bigger particle size (fit to the request). 3. C18 RP18 - 5µm – 250 x 4,0mm. 100A pore , 350m2/g Area , 21,0% carbon. pH bwt 2,0 to 7,5. Equivalent to a HPLC Elite Le Chrom. *Asahipak ODP-50 4E (5 um, 4.6 x 250 mm, 250 A pore, pH 2-13) 4. C8 RP8e - 5µm – 125 x 4,0mm. 120A pre size , 330m2/g Area , 11,2% carbon pH bwt 1,5 to 10,5. *RSpark RP18-413 (3.5 um, 4.6 x 150, no functional group – but will work like a reversed phase mode, 200 A pore) Only reversed phase column that is usable under pH bt 1-13. But since this is not modified with any carboxyl functional group, the separation performance may differ from expected RP analysis. If the customer is not using mobile phase <2, Asahipak C8P-50 4D (5 um, 4.6 x 150 mm, 250 A pore, pH 2-13) can be recommended. This has octyl functional group. Also this has bigger particle size (fit to the request). 5. C8 RP8 – (5µm) 125 x 4,0 mm, 100A pore size , 350m2/g Area , 12,5% carbon. pH bwt 2,0 e 7,5. Equvalent to HPLC EliteLechrom. *Asahipak C8P-50 4D (5 um, 4.6 x 150 mm, 250 A pore, pH 2-13) 6. Silica Coluna with a ciano group - CN; 125x4mm - 5um: , 100A; Volumen= 1,25 ml/g; Área= 350m2/g;% carbon= 6,6%; Cobertura da superfície: 3,52 umol/m2; Efficiency : > 40.000 N/m – 15.000 N/m; pH: 2,0 a 7,5.

*Silica 5CN 4D (cyanopropyl functional group, 5 um, 4.6 x 150 mm, 100 A pore, pH 2-8, >48,000N/m)

7. Pré-colunas C18. sílica 5µm, 120A pore , 330m2/g Area específica, 3umol/m2 with 17% carbon . pH bwt 1,5 e 10,5.

8. Pré-colunas C18. sílica 5µm, 120A pore , 330m2/g Area específica, 3umol/m2 with 11.2% carbon . pH bwt 1,5 e 10,5.

*Are they talking about the Guard column? –also we do not have a guard (nor pre-column) for silica based columns…and I don’t think any silica column can work under pH 1.5…

9. Pré-colunas CN with ciano group 5µm, poro: 100A; Volumen: 1,25 ml/g; Área: 350m2/g; % Carbon: 6,6%; Eficiência: > 40.000 N/m – 15.000 N/m; bwt pH: 2,0 a 7,5.

*Again, we do not have Pre-column. Also for CN, we do not have a guard.

 
 
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