galactose & glucose separation, sugar in meat

Record: 287

Category: Specifications
Product: SUGAR
Sample:

Question

Answer
1.Regarding the galactose and glucose separation, SP0810 will be a good separation, compared to other SUGAR series columns.
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2.For the sugar analysis in meat, it does require removal of impurities such as protein and salt.
One of the recommended method is following:

Homogenize the sample.
Weigh 0.5~5 g of the sample into 50 mL beaker.
Add 30 mL water and sonicate for 30 min.
*If the sample is acidic, add 10 % (w/v) NaOH to neutralize the sample.
Add water to bring up the volume 50 mL.
If there is any undissolved components, filtrate to remove the residuals.
Inject 5~10 mL of the sample into ion exchange SPE to remove the salt.
Filter the sample with 0.45 um filter membrane.

 
 
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