HPLC Column Selection

Record: 406

Category: Column Selection
Product: SP0810
Sample:

Question
. I am just beginning to research specific HPLC columns and my knowledge of detail is minimal. We are going to be running both urine and plasma samples to quantify four sugars; sucrose, lactulose, rhamnose, and sucralose. We are currently planning on utilizing HPLC with an electrochemical detector. Throughout the literature there is a lack of consistency as to the type of column and analysis of these sugars. Some sources have been able to run all four of the sugars together, while others have run the sucralose separately. If we could run all four simultaneously that would clearly be the more efficient option. From what I understand the sucralose has a longer retention time than the other sugars, it was suggested we try a 5% acetonitrile mobile phase if the sucralose RT is too long. Another concern is the retention times of sucrose and lactulose and that of lactulose and rhamnose on varying columns.

Answer
I would suggest taking a look at our SP0810 column.
Its separation is based on size exclusion and ligand exchange (Pb2+ ligand). The mobile phase required is simply water. You may also add ACN to optimize the elution time also. In below application, you can see the separation of sucrose (peak #4) and rhamnose (peak #7). Rhamnose will elute at almost the same retention time as galactose (peak #8). Depends on the concentration of those two sugars, since those two elute out close, you might observe some overlap of those two peaks.
http://shodex.net/index.php?seitenid=1&applic=1009

As you mentioned, sucralose will elute at later time, ~15 min using SP0810.
http://shodex.net/index.php?seitenid=1&applic=874

We have a technical notebook available for sugar (food) analysis. This might be a useful information for you.
http://shodex.net/index.php?seitenid=1&applic=874

 
 
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