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Hyaluronic acid

Record: 534


Category: Specifications
Product: SB
Sample:


Question
Now I connected new SB807HQ columns and running PBS buffer.
I saw the max. pressure of columns is 0.5Mpa(5bar) in column manual.
I think too low pressure because it’s only flow 0.1 ml/min(5bar).
Could I increase flow rate? or Is it correct? Folw rate: 0.25ml/min, with column:9bar, without column:5bar

 
 

Answer
Some of the things you might want to check are:
Check the connecting tubings and screws – are they new?
If not new, may be some salt deposition?

Mobile phase. What eluenet are you using? Filtered well?
Is your degassor working properly?

 
 
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