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Interaction

Record: 525


Category: Column Problem
Product: KF
Sample:


Question
I am injecting tricarbanilated cellulose molecules that have some SO3- charges. Eluent is THF.
Please let me know if there is something I can do to avoid this interaction with the columns.

 
 

Answer
Would you please find out the following conditions?
Concentration and the injection volume of the sample (tricarbanilated cellulose).
Column temperature.

Recommended concentration is less than 0.5% w/v, 50-100 uL.
Temperature ~40oC.

 
 
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