IPEN information

Record: 368

Category: General
Product: non-shodex C18
Sample: 18F and 18F-FAC

Question
I am developing a new radiopharmaceutical for PET / CT, the 18F-FAC (fluoroacetate), but I’m not getting results in HPLC. I have used a C18 column with the solvent ethanol / water (10/90), but there is no separation of the 18F and 18F-FAC.

Answer
Are you follwing a method used by someone?
Since F and F-FAC are probably in ionic state under your mobile phase, I think they are not retained on RP system.
I am wondering if you can use buffer to control the pH of mobile phase.
e.g. http://shodex.net/index.php?seitenid=1&applic=1407
By using acidic condition, two components will become non-polar, suitable for the RP analysis.
If controlling the pH does not work and if you need to work with a C18 column, next step might be use of ion-pair reagent.
I would recommend you to try changing the mobile phase pH first. Please let me know if you have further question.

 
 
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