KW column storage Thank you for your interest in Shodex

Record: 784

Category: Specifications
Product: KW
Sample:

Question
Can you please tell me what is the long term storage procedure for Shodex KW 802.5 columns ?

Answer
Please follow the below steps:

You may store the column with 100% Water, but if you are worried about bacterial growth, storing the column with 20% MeOH is recommended over sodium azide solution, since NaN3 is a basic eluent and KW series is not recommended to use under basic conditions.

For the long term storage, using the following method is the best way.
It’s written in the manual…but I’m sorry it was only in Japanese.
The translation is in below and you may also refer to the figure on pg. 8.
http://shodex.net/data/downloads/PROTEIN_KW-800series_EJ_No.615-5.pdf

After cleaning the column with DI water and 20 % MeOH.
1. Remove the exit end of the column from the HPLC system, attach a 1/16 inc 0.8 mm i.d., 50 cm Teflon tubing.
2. Start the pump and once observing eluent flow from the Teflon tubing, stop the pump and place the Teflon tubing in a 100mL solvent resorvoirs which contains 80 mL of the solvent.
3. Remove the entrance side of the column from the HPLC system, screw the end-cap and keep the column (with eluent) at a place with small temperature fluctuations. It is also recommended to change the reserving eluent one a month.

For your information, below is the recommended cleaning steps for KW series column.
When observing the high pressure, reverse the flow and introduce the eluent at slower flow rate.
When suspecting protein adsorption, use ~0.3M NaCl solution to clean the column.
*do not leave NaCl solution in the column for a long time period since it may cause rust to the column housing.

When suspecting organic substance adsorption, clean with DI water first, and then clean with organic solvent such as MeOH.

 
 
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