Shodex Column Recommendation for Triglycerides, Diglycerides, Monoglycerides, and Methyl Esters

Record: 569

Category: Column Selection
Product: GF-310
Sample: Methyl Esters

Question
A customer is looking for a suitable column to simultaneously analyze triglycerides, diglycerides, monoglycerides, and methyl esters using HPLC. They found an application referencing Shodex Asahipak GS-310 7G but cannot locate ordering information. Is this column still available, and what is the recommended alternative?

Answer
The referenced application is appropriate for this type of analysis; however, the Asahipak GS-310 7G column is no longer available.

The current recommended replacement is Shodex Asahipak GF-310 HQ. This column is designed for size exclusion chromatography (SEC/GPC) and is suitable for the separation of lipid classes such as triglycerides, diglycerides, monoglycerides, and methyl esters based on molecular size differences.

SEC/GPC provides an effective approach for this type of analysis, as it allows separation of lipid components without relying on polarity differences, which can be challenging when analyzing structurally similar compounds.

It should be noted that triglycerides, diglycerides, monoglycerides, and methyl esters each include a wide range of compounds with varying structures and molecular weights. The optimal method and separation performance will depend on the specific target analytes and required resolution.

For more tailored recommendations, including method optimization, please contact Shodex technical support with details on the sample composition and target compounds.

Recommended Applications:

• Triglycerides
• Nonylphenol Ethoxylates (GF-310 HQ)
• Conjugated Lipid (L-α-Phosphatidylethanolamine)

Additional Comments

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