Shodex Columns for Mono- and Dicarboxylates Analysis by LC–MS

Record: 885

Category: Column Selection
Product: NH2P-40 3E
Sample: mono- and dicarboxylates

Question:
Which Shodex column is recommended for separating small mono- and dicarboxylates in LC–MS without using mineral acids or salts?

Answer:
Shodex offers options for small carboxylate and dicarboxylate analysis, depending on the detection method:

• Organic acid analysis (non-MS):
  The DE-413 column can be used for separating dicarboxylic acids.
  Application – Dicarboxylic Acids (DE-413)
  However, this method uses non-volatile eluents and is not compatible with LC–MS.

• LC–MS compatible analysis:
  The NH2P-40 3E is recommended for LC–MS applications.

  • Mode: Polymer-based amino HILIC

  • Particle size: 3 µm, 4.6 mm I.D. × 250 mm

  • pH stability: 2–13

  • Mobile phases: Compatible with volatile eluents (e.g., acetonitrile/water with ammonium formate or ammonium acetate)

  • Provides good retention of polar organic acids without requiring non-volatile mineral acids or salts.

For LC–MS applications, use NH2P-40 3E. For general organic acid separations where MS compatibility is not required, DE-413 may be suitable. 

Additional Comments

Please contact Shodex technical support for further guidance based on your sample and system setup.

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