Shodex Column Recommendation for Smaller ID Equivalent of KW-803

Record: 820

Category: Column Selection
Product: KW403-4F
Sample: N/A

Question
I’m currently using a Shodex KW-803 column. Is there a smaller inner diameter (I.D.) version available?

Answer
Yes — if you’re looking for a smaller I.D. version of KW-803, the recommended option is KW403-4F.

Both belong to the same OHpak KW series for aqueous size-exclusion chromatography (SEC/GFC), designed for water-soluble proteins and biopolymers.

The KW403-4F offers the same separation mode and polymer-based hydrophilic packing but in a 4.6 mm I.D. format, ideal for lower flow rates and smaller sample volumes.

• Recommended column: OHpak KW403-4F (4.6 mm I.D. × 300 mm, 3 µm)
• Mode: Aqueous SEC (GFC)
• Typical samples: Proteins, peptides, antibodies, enzymes
• Eluent: Phosphate buffer or 0.1 M NaCl solution (neutral pH)
• pH range: 3 – 10

So, if you’re transitioning to analytical-scale or LC–MS-compatible SEC, KW403-4F is the best smaller-I.D. equivalent to KW-803.

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