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Pressure issues after amino acid analysis

Record: 721


Category: Column Problem
Product: NH2P
Sample:


Question
amino acid by NH2P-40 2D. after ~ 50 injections 10 umL sample pH ~5, change in peak Rt. no change in back pressure.

 
 

Answer
Please wash with 0.1N NaOH. Try at 0.5 mL/min for about 1 hour.

 
 
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