Protein KW-802.5

Record: 315

Category: Specifications
Product: KW
Sample:

Question
I work for Bayer Healthcare in Seattle and we are currently working with your column (KW-802.5 8.0mmIDX300mmL) to implement into one of our quality control assays. This column type is said to have a maximum pressure of 5.0MPa (725psi), but some analysts in one of our other locations are claiming they have been getting between 800-1000psi. I just wanted to know if that was even possible? I’m looking at the CofA for one particular column that says the pressure it got was 2.1MPa (about 305psi). This is the same column they are getting 800-100psi on it. How is that possible and what happens if it is possible? They also said that the run looked fine with the pressure that high. Any information about this would be very helpful.

Answer
It is interesting to hear that a KW column is working well at such an elevated pressure.
I am wondering if there is a possibility that the other lab is using two KW columns in series.
Obviously if you connect two columns, the back pressure will double, but this is an acceptable usage method with max pressure (5 MPa x 2 = 10 MPa).

I do not think we have a data taken at an elevated pressure conditions. The maximum pressure stated in the operation manual is that the condition we guarantee the column works. Thus it does not mean that the column will break suddenly when used with back pressure over 5 MPa. It only means that we cannot guarantee the performance under the situation. We do hear customer use columns outside of guaranteed pH range and still give them good results. If your HPLC system does not stop at this pressure range, the column might be used. Generally speaking, using the column over guaranteed maximum pressure will shorten its column life.

 
 
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