Protein KW column and Your shodex US contact

Record: 139

Category: Column Selection
Product: GPC
Sample: protein

Question
For your protein analysis, we recommend our KW400 series columns.
The column can be used in the pH range 3-10 and is tolerant for up to 100% acetonitrile.
However, unfortunately currently we do not have any application data using KW400 for the protein analysis with volatile eluent.

The suitable flow rate of KW400 column is 0.3 mL/min (or <0.2 mL provides better resolution) and with this flow rate, the run time is about 15 min. Below is technical note discusses more details on KW400 columns. http://shodex.net/data/downloads/Shodex_Technical_Notebook_5_KW400.pdf If you are unsure about which KW400 series is suitable for your analysis, please let me know the molecular weight range of your proteins. I’ll be happy to assist you deciding which one.     Answer
I need assistance choosing the correct
column. We need a size-exclusion column to be coupled with a mass spectrometer for analysis of proteins, so there are three requirements.
First, it has to be able to tolerate acidic conditions of about pH=3.
Second, it must tolerate mixtures of water and organic solvents (such
acetonitrile) up to about 50%, but this is flexible. Finally, the flow rates must be low enough to be compatible with mass spectrometry, so ideally we would like to flow at about 100-600 uL/min and have runs finish in 30 minutes or less.

 
 
Additional Comments
I think KW400 series columns are usable with the following reasons:

1, The usable pH of KW400 is 3 – 10.
2, KW400 series columns are usable with 0 to 100% of acetonitrile and methanol 3, The suitable flow rate of KW400 columns is 0.3mL/min 4, The time for one analysis is about 15min.

Please note we do not have the application data of KW400 for the analysis of protein with the volatile eluent condition at present.
So, could you please add this our situation?
And please ask the approximate molecular weight of the target proteins to select the suitable column.

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